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Literature summary extracted from
- Immobilisation of bovine enterokinase and application of the immobilised enzyme in fusion protein cleavage (2008), Bioprocess Biosyst. Eng., 31, 173-182.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.4.21.9 | biotechnology | EK is immobilised on hexamethylamino Sepabeads or on amino-modified paramagnetic microspheres. 50% of activity remains after immobilisation | Bos taurus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.9 | Bos taurus | - |
- |
- |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.21.9 | The free enzyme has a half life of 8 days at 23°C. The immobilised enzyme has a half life of 29 days at 23°C. The immobilised and glutardialdehyde treated enzyme has a half life of 36 days at 23°C. The free enzyme has a half life of 85 days at 4°C. The immobilised enzyme has a half life of 10 month at 4°C. | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.9 | Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O | - |
Bos taurus | Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine | - |
? |  |
| 3.4.21.9 | MUC1-IgG Fc + H2O | fusion protein | Bos taurus | MUC1 + IgG Fc | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.9 | enteropeptidase | EK | Bos taurus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.9 | 25 | - |
immobilised enzyme on hexamethylamino Sepabeads or paramagnetic microspheres | Bos taurus |
| 3.4.21.9 | 33 | - |
free enzyme | Bos taurus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.9 | 15 | 40 | - |
Bos taurus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.9 | 6 | - |
immobilised enzyme on paramagnetic microspheres | Bos taurus |
| 3.4.21.9 | 8 | - |
free enzyme | Bos taurus |
| 3.4.21.9 | 9 | - |
immobilised enzyme on hexamethylamino Sepabeads | Bos taurus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.9 | 5 | 10 | - |
Bos taurus |
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Release 2023.1 (January 2023)
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